Biofertilizers are substances that enhance soil fertility by harnessing microorganisms that form symbiotic relationships with plants, providing essential nutrients like nitrogen and phosphorus. They contribute to soil health, reduce environmental pollution, and minimize the need for chemical fertilizers. Our study reaffirmed previous findings regarding the antifungal properties of Eclipta alba extracts against sorghum pathogens. Specifically, methanolic fractions of E. alba exhibited potent antifungal effects against harmful sorghum grain mold pathogens such as F. thapsinum, A. alternata, E. sorghinum, and C. lunata. Purification of the methanol E. alba extract through column chromatography enhanced its antifungal potency by removing nonpolar components. Phytochemicals like eclalbasaponin II and wedelolactone, found in higher concentrations in methanolic fractions, significantly bolstered the antifungal activity against targeted sorghum pathogens. This research underscores E. alba's potential as a biofertilizer and biopesticide due to its rich array of active phytochemicals. The susceptibility of sorghum to various pathogens poses a significant challenge to its cultivation, with fungal diseases causing substantial yield losses worldwide. This study emphasizes the importance of exploring natural alternatives like E. alba extracts in combating sorghum diseases and enhancing agricultural productivity.